The intrinsic apoptosis pathway is activated inside the cell by the Bcl-2 protein family and is independent of receptor signal transduction 8, 9. These pathways are also known as mitochondrial and death receptor pathways, respectively 8. Apoptosis is mediated by an intrinsic or extrinsic pathway based on the origin of the apoptotic stimulus. Targeting apoptosis avoidance, a major feature of cancers, is the most effective nonsurgical strategy for the treatment of cancers because it is not specific to the cause or type of cancer 8. However, adversary side effects, poor bio-distribution, and high toxicity to non-cancerous cells limit the use of DOX 6, 7. Doxorubicin (DOX) is an efficient chemotherapeutic agent that is widely used for the treatment of various cancers 5. Consequently, high chemotherapeutic doses that are adversary to human health are the only options to treat the mucosal and epithelial membranes of the affected tissues. Surgical resection and systemic chemotherapy can affect drug efficacy. Tumor cells in the ovary can metastasize to the lymphatic and circulatory systems if not blocked by the anatomical barriers 4. In addition, the administration of chemotherapeutic agents and multi-drug resistance are accompanied by severe side effects that cause negative gynecological and obstetric outcomes 2, 3. Although the cervical cancer screening program has reduced the incidence and mortality of cervical cancer, the incidence among young women remains a public health problem 1. glyptostroboides floral cones, especially the DME extract, which possesses several anticancer components, as determined by GC–MS analysis, could a potential natural anticancer agent.Ĭervical cancer affects many women in developing countries and has a high mortality rate. Our results suggest that the extracts of M. Furthermore, RT-qPCR and western blotting were used to quantitate the relative RNA and protein levels of the cell death pathway components, respectively. The number of cells at the sub-G1 phase, which indicates apoptotic cells, was increased approximately fourfold upon treatment with the DME and CE extracts compared with that in the negative control. Further, the sub-G1 population was determined by FACS analysis. The viability of COS-7 cells was found to be 12-fold higher than that of the HeLa cells under the administration of 50 µg/ml of the DME extract. The anticancer effect was evaluated by using the Cell Counting Kit-8 assay. HeLa cervical and COS-7 cells were used as a cancer cell model and normal cell control, respectively. glyptostroboides floral cones was subjected to methanol extraction, and the resulting extract was further partitioned by liquid–liquid extraction using the organic solvents n-hexane, dichloromethane (DME), chloroform, and ethyl acetate in addition to deionized water. Latest Winrar and extract password protected files without error.This study was undertaken to investigate the anticancer effects of organic extracts derived from the floral cones of Metasequoia glyptostroboides. TO WIN USERS: If RAR password doesn't work, use this archive program: RAR Expander 0.8.5 Beta 4 and extract password protected files without error. TO MAC USERS: If RAR password doesn't work, use this archive program: Texture rendering imperfections and some glitches that may occur can be avoided by adjusting the Direct3D and OpenGL preview options.Īll things considered, Metasequoia seems to be a worthy alternative to more complex utilities of its kind, especially thanks to the ease of use coupled with a good support for a variety of formats this software is able to work with. There are many customizations that can be made to the application from the 'Configuration' area, so you can assign, for instance, various actions to mouse buttons and key combinations. Each selected portion of the design can be fixed, stored or restored when needed, as well as fit to view or set as center of rotation. The editing function that Metasequoia is equipped with are rather basic, but, nonetheless, cover all the necessary actions for handling objects, material faces and vertices. There is a wide variety of objects that you can select and insert in the design, as well as some tools that are meant for modifying the model you are working on. Each of these styles comes with its commands for editing the loaded model, setting the appropriate lighting and more.
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